Reporter

Part:BBa_K4007000:Design

Designed by: Jonatan Joel Aliaga Rodriguez   Group: iGEM21_Bolivia   (2021-10-05)


mRFP1 violet variant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Added an aditional stop codon at the end of the coding sequence


Source

The source of the part will be its sequence which was retrieved from Addgene "Overcoming chromoprotein limitations by engineering a red fluorescent protein" page.

References

Bao, L., Menon, P. N. K., Liljeruhm, J., & Forster, A. C. (2020). Overcoming chromoprotein limitations by engineering a red fluorescent protein. Analytical Biochemistry, 611, 113936. https://doi.org/10.1016/j.ab.2020.113936